The use of epifluorescence microscopy and fluorescent dyes for visualization of Oxyuris equi eggs.

This study presents a new method for visualization of Oxyuris equi eggs collected by means of a clear adhesive tape applied to the perianal region of horses. The obtained results indicate that this protocol permits a quick, easy, clear and selective visualization of Oxyuris equi eggs even in the presence of feces and other material. Another advantage of this method is that it can be used with fluorescent dyes solubilized in water, which will stain biologic material without dissolving or altering the adhesive tape and is also environmentally safe. Other dyes currently used for staining biologic materials use organic solvents, which may be combined with acids or bases in their formulation, preventing their use with the tape method.

A new methodology for the visualization of latent fingermarks on the sticky side of adhesive tapes using novel fluorescent dyes.

Three novel fluorescent dyes were evaluated for the detection of latent fingermarks on different types and colors of adhesive tapes. Compared with the conventional reagents used to reveal latent fingermarks on these surfaces, these new fluorescent dyes have many advantages. They are highly selective to fingermarks, require only a simple procedure, do not need pre- or post-treatment, have high thermal and photochemical stability, are low in cost and use only water as a solvent. In addition, the emitted fluorescence creates a sharp contrast with the fingermark surface, meaning the fingermarks can be clearly visualized and photographed when excited with longwave ultraviolet light (365nm).